PCR Supplies
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
| Physical Form | Liquid |
|---|---|
| pH | 7 |
| Chemical Name or Material | Saline-Sodium Citrate |
| Grade | Molecular Biology |
| DNase | DNase free |
| Recommended Storage | RT |
| Concentration | 2.9 to 3.1 M |
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 2X |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Starting Material | DNA |
| For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 2X |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Thermo Scientific™ Phusion Human Specimen Direct PCR Kit
Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phire Hot Start II DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Direct PCR Master Mix |
| Sample Type | Plant |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| Starting Material | DNA |
| For Use With (Application) | Genotyping,Hot-start PCR |
| PCR Method | Direct PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
| Quantity | 4 x 1.5 mL |
|---|---|
| For Use With (Application) | Standard PCR |
Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Flash II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Color | Colorless |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)
This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | High |
| Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Product Type | High-Fidelity PCR Kit |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion U Hot Start DNA Polymerases
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Product Type | Hot Start DNA Polymerase |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
| Shipping Condition | Room Temp or Wet Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate components |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |