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Gibco™ AAV-MAX Helper-Free AAV Production System Kit 
The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production.
Supplier: Gibco™ A51217
Description
The Gibco AAV-MAX Helper-Free AAV Production System is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.
Features of the AAV-MAX Helper-Free AAV Production System include:
- Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
- Suspension-based system has scalable protocols from shake flask to bioreactor scale
- Simplified workflow—streamlined protocol using helper virus-free triple transfection
- Clonal, 293F-derived, high-producer cell line
- Robust transfection protocols are adaptable to large-scale transfection
The components included in the AAV-MAX System Kit are:
- 1 vial of Viral Production Cells 2.0
- 1 L of Viral Production Medium
- 1 AAV-MAX Transfection Kit for 1 L of production
- 100 mL Viral-Plex Complexation Buffer
- 100 mL AAV-MAX Lysis Buffer
All components are also available for purchase separately.
Specifications
| • 1 vial Viral Production Cells 2.0 • 1 L Viral Production Medium • 1 AAV-MAX Transfection Kit for 1 L production • 100 mL Viral-Plex Complexation Buffer • 100 mL AAV-MAX Lysis Buffer • 100 mL GlutaMAX Supplement Storage conditions vary |
|
| Kidney (Embryonic) | |
| AAV Production System | |
| AAV Production | |
| Sufficient for 1 L of Culture |
| Viral Production Cells 2.0 | |
| Suspension Cell Culture | |
| Sterile | |
| AAV-MAX |
For Research Use Only. Not for use in diagnostic procedures.