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Molecular Probes™ Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate 
Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate
Supplier: Molecular Probes™ A32360
Description
Alexa Fluor™ 488 Streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.Important Features of Alexa Fluor™ 488 Streptavidin Conjugates:
Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Specifications
| 30 μg/mL | |
| 0.5 mL | |
| Wet Ice | |
| Alexa Fluor™ | |
| Alexa Fluor Dyes |
| Alexa Fluor 488, FluoroNanogold | |
| Store in refrigerator (2–8°C) and protect from light. | |
| Liquid | |
| Streptavidin Conjugate (fluorescent) |
For Research Use Only. Not for use in diagnostic procedures.