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Invitrogen™ RNAqueous™-96 Total RNA Isolation Kit 
The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
Supplier: Invitrogen™ AM1920
Description
The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous™-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.
Using the RNAqueous™-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous™-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).
Accessory product
The RNAqueous™-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
Specifications
| • 50 mL Nuclease-free Water; store at room temperature • 50 mL Elution Solution; room temperature • 2 Filter Plates; room temperature • 2 Collection Plates; room temperature • 2 Adhesive Plate Sealers; room temperature • 2 Plate Lids; room temperature • 50 mL Lysis/Binding Solution; 4°C • 175 mL Wash Solution (concentrated); 4°C • 53 mL Rebinding Mix; 4°C • 12 mL DNase I Buffer; -20°C • 1.25 mL DNase I; -20°C |
|
| ≥50 μL | |
| Total RNA | |
| MultiPROBE™ II HT | |
| 192 Preps | |
| Cells: ≤106 Tissue: ≤1.5 mg |
| Silica Plate | |
| Cells, Tissue | |
| High-throughput Compatible | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Box 1: Dry Ice Box 2: Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.