Invitrogen™ SUPERase·In™ RNase Inhibitor (20 U/μL) 

Description

SUPERase·In™ RNase Inhibitor is a protein-based inhibitor of nonhuman origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1. SUPERase·In RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase·In is the most effective RNase inhibitor available, providing a higher level of protection against degradation. SUPERase·In does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or Taq DNA polymerase. Additionally, SUPERase In is active up to 65°C and over a pH range of 5.5 to 8.5.

• Completely removes RNase contamination from glass and plastic surfaces
• Excels at removing high levels of RNase contamination where similar products fail
• Proven effective at removing high concentrations of dried-on RNase A
• Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free

Unit definition
SUPERase·In RNase Inhibitor at 1U/μL will block the degradation of 0.1μg/μL labeled RNA by 2.5pg/μL of RNase A, 2.5pg/μL of RNase I, and 0.0075U/μL of RNase T1, for four hours at 37°C, in 20mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA. Analysis is by denaturing PAGE. SUPERase·In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.

If using this product with standard antibody purification methods, please contact technical support to discuss experimental design.

Gene Expression Analysis and Genotyping, In Vitro Transcription, PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription