Mouse Anti-Actin, Monoclonal (Clone C4)

Description

• Chicken gizzard actin has been used as the immunogen
• Immunoblots demonstrate specificity directed towards all actins ranging from yeast, to flies, to mouse, to man
• Actins are: skeletal alpha-actin, cardiac alpha-actin, smooth muscle gamma actin, cytoplasmic beta-actin, cytoplasmic gamma actin as well as Dictyostelium discoideum and Physarum olycephalum actins
• Epitope for the antibody lies between residues 18 to 40, a highly conserved region of the molecule
• Can also be used for cells cultivated on slides, for frozen tissue sections and as a loading control for western blots

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly