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Cytiva bacteria genomicPrep Mini Spin Kit
Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria
Supplier: Cytiva 28904259
Includes: illustra bacteria genomicPrep Mini spin columns prepacked with novel silica membrane; Lyophilized proteinase K powder; Lysis solutions, wash and elution buffers; 2mL Microcentrifuge collection tubes; a full booklet with a detachable, quick reference protocol card
Description
The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.
The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.
- Total DNA extraction time under one hour with reduced lysis time
- Can be used to purify 4 to 8μg of genomic DNA from 2 × 109 bacteria cells with input amounts ranging from 1 × 109 to 4 × 109 bacteria cells
- Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
- Validated in several downstream applications including RT PCR, long PCR, and restriction digest
- Color-coded caps for ease of use
Specifications
| illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria. | |
| 30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes | |
| illustra bacteria genomicPrep Mini Spin Kit | |
| 2 x 109 cells [A600 = 2.0] |
| For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping | |
| 250 Preps | |
| 1.7 | |
| 40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria] |