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Invitrogen™ C1q Human ELISA Kit DFS Item

Sandwich ELISA Kit

Supplier:  Invitrogen™ BMS2099FIVE

Catalog No. BMS2099FIVE


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Description

Description

The Human C1q ELISA quantitates Hu C1q in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C1q. Principle of the method The Human C1q solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1.
Specifications

Specifications

P02745
0.08 ng/ml
ELISA Kit
Human
712
4.2%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
5 x 96 Tests
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
Human
4 hr. 30 min.
0.9-60 ng/ml
Biotin
Plasma, Serum, Supernatant
Colorimetric Microplate Reader
C1QA
4.7%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
SDS
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