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Molecular Probes™ CellROX™ Orange Flow Cytometry Assay Kit DFS Item

CellROX™ Orange Flow Cytometry Assay Kit

Supplier:  Molecular Probes™ C10493

Catalog No. 501121510


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Description

Description

The CellROX™ Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Orange Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX™ Reagents and Kits.

The CellROX™ Orange Flow Cytometry Assay Kit features:

  • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
  • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
  • Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

    The CellROX™ Orange Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 545/565 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Red Dead Cell Stain, oxidatively stressed and nonstressed cells are reliably distinguished from dead cells by flow cytometry.
    • Specifications

    Specifications

    Tube
    100 assays
    CellROX orange: 545/565, SYTOX red: 640/658
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    For Research Use Only. Not for use in diagnostic procedures.