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Thermo Scientific™ DNA Polymerase I (10 U/μL)
DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.
Supplier: Thermo Scientific™ EP0042
Description
DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'→3' synthesis of DNA. The enzyme also exhibits 3'→5' exonuclease (proofreading) activity, 5'→3' exonuclease activity, and ribonuclease H activity.
Highlights
- Incorporates modified nucleotides(e.g. biotin-, digoxigenin-, aminoallyl-, fluorescently-labeled nucleotides)
- Active in multiple buffers, including restriction enzyme, PCR, and RT buffers
Applications
- DNA labeling by nick-translation in conjunction with DNase
- Second-strand synthesis of cDNA in conjunction with RNase
Specifications
| DNA Polymerase I | |
| DNA Polymerase I | |
| 2,500 units | |
| 10 U/μL |
For Research Use Only. Not for use in diagnostic procedures.