Thermo Scientific™ DNase I, RNase-free (1 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Supplier: Thermo Scientific™ EN0521

Catalog No. FEREN0521

$103.58 / Each

Quantity

Description

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.

In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides

Highlights

Recombinant enzyme
Purified from non-animal host with a lower level of intrinsic RNases

Applications

Preparation of DNA-free RNA
Removal of template DNA followingin vitrotranscription
Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
DNA labeling by nick-translation in conjunction with DNA Polymerase I
Studies of DNA-protein interactions by DNase I, RNase-free footprinting
Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications

Concentration	1 U/μL
Compatible Buffer	Reaction Buffer
Product Type	DNase I RNase Free

Enzyme	DNase
Quantity	1,000 units

For Research Use Only. Not for use in diagnostic procedures.