Invitrogen™ Dynabeads™ In Vitro Transcription and RNA Purification Kit

Description

The Dynabeads In Vitro Transcription and RNA Purification Kit offers an innovative technology for simple, flexible, and automation-ready mRNA production and is particularly suitable where high quality and scalability are required, such as during the discovery phase of RNA-based vaccine and therapeutic development. This technology can help speed up the screening of hundreds of constructs that may be required to identify a final drug candidate, in scalable quantities to match your requirements.

Features of the Dynabeads In Vitro Transcription and RNA Purification Kit include:
• Flexibility—choose DNA template for IVT mRNA with greater flexibility of design and amplification
• Efficiency—reuse DNA template and beads at least six times to reduce the cost and eliminate/minimize plasmid prep hands-on time and risk of transfer of bacterial host DNA
• Scalability—swap between manual and automation-ready workflows to match required scale (micrograms to grams of mRNA) and throughput
• Quality—ensure a safe and flexible supply of magnetic beads to navigate from RNA-based drug discovery to commercial manufacturing without incurring extra time or costs from switching technology or raw material

The biotinylated template is made by PCR amplification of the target sequence in a plasmid construct or in a synthetic DNA construct using a biotinylated forward primer located 30–100 base pairs upstream of the T7-promoter and a non-biotinylated reverse primer. As an alternative to PCR amplification, a linearized plasmid can be biotinylated by a fill-in reaction of biotin-dUTP in the 5' overhang sequence, provided the correct plasmid design. The biotinylated template is then immobilized directly to Dynabeads Streptavidin for In Vitro Transcription without the need for prior purification. The bead-bound template is used directly in the IVT, then removed from the synthesized mRNA by magnetic separation. The template can be reused at least six times in consecutive IVT reactions.

Crude RNA from the IVT step is mixed with the beads and buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet and discarding the supernatant.

To learn more about other products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics visit: thermofisher.com/dynabeadsmrna