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Edvotek™ Radial Immunodiffusion Kit
Introductory-level experiment quantitatively determines the unknown concentration of an antigen.
Supplier: Edvotek™ EMD273
Includes:
- Antibody solution
- Standard antigen solution
- UltraSpec-Agarose™ powder and buffer powder
- Unknown concentration of antigen
- Sleeve of Petri dishes
- Two 10mL pipets
- 10 well cutters
- 80 transfer pipets
- 70 microtest tubes
- Graph paper template
- Practice loading solution
Requires :
- Automatic micropipets and tips (5 to 50μL)
- Pipet pumps for 10mL pipets
- Ruler
- Plastic box or dish
- Plastic wrap
- Foil
- Paper towels
- Distilled water
- Hotplate, Bunsen burner or microwave
- 400 to 600mL beaker or Erlenmeyer flask
- 150mL beaker or flask
- Water bath (55°C [131°F])
- 250mL graduated cylinder
- 37°C (98.6°F) incubation oven
Description
- Antibody is incorporated into liquefied agar and allowed to gel; antigen is introduced by circular wells
- Sensitive and quantitative
No experiment components contain human serum.
Specifications
| Radial Immunodiffusion Kit | |
| Instructions, antigen and antibody, petri plates, pipets, well cutters, agarose, buffer, microtest tubes | |
| 10 Lab Groups |
| To quantitatively determine the unknown concentration of an antigen | |
| Refrigerated; Store at: 4°C; 39°F |