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MilliporeSigma™ EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
Specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipitation and enables the use of a broader range of antibodies, eliminating the need to purchase different kits for different antibody isotypes.
Supplier: MilliporeSigma™ 1710461
Includes:
- 10X Glycine
- Nuclei Isolation Buffer
- 10X PBS
- SCW Buffer (Sonication/ChIP/Wash)
- Magna ChIP Protein A/G Magnetic Beads
- Low Stringency IP Wash Buffer
- ChIP Elution Buffer
- Protease Inhibitor Cocktail
- Proteinase K Solution
- Anti-Trimethyl-Histone H3 (Lys4)
- Normal Rabbit IgG
- Control PCR primers
Requires :
Reagents
- Cells, stimulated or treated as desired
- Antibody of interest for chromatin immunoprecipitation
- 37% Formaldehyde
- qPCR reagents, such as SYBR™ Green Master Mix
- 2% Agarose gel
- 100 bp DNA Ladder
- 6X gel Loading buffer
- DNase- and RNase-free sterile H2O
Description
- Robust ChIP from as few as 10,000 to as many as 1,000,000 cells
- Protocols and reagents for generation of chromatin from range of input sample type
- Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies
- Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment
- Single buffer system for sonication, chromatin IP, and wash
- Perform analysis of enrichment without additional purification after cross link reversal
- Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray
- Includes positive and negative control antibodies and assay against known positive locus
Specifications
| Store components at the temperatures indicated on the labels | |
| To enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads | |
| 24 Assays | |
| Kit components are stable for 1 year from date of shipment when stored as directed |