Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Description

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Usage Recommendations

• Use fresh 1X TAE both for the gel and for the electrophoresis run
• To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

• 40mM Tris
• 20mM acetic acid
• 1mM EDTA
• pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.