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Thermo Scientific™ Biotin-11-dUTP Solution (1 mM)
Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.
Supplier: Thermo Scientific™ R0081
Description
Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.
Applications
• Enzymatic non-radioactive labeling of DNA by PCR
• Nick-translation
• cDNA synthesis
• Random primed labeling
• Primer extension
Biotin-11-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA Polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment, exo (EP0421)
• Klenow Fragment (EP0051)
• DNA Polymerase I (EP0041)
Specifications
| 1 mM | |
| Liquid | |
| Direct Labeling | |
| 50 μL | |
| cDNA Synthesis, Non-Radioactive Labeling |
| Biotin-11-dUTP Solution, 1 mM (50 μL) Store at –20°C. |
|
| DNA Polymerase | |
| Biotin | |
| Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.