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Thermo Scientific™ Genomic DNA Purification Kit
Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.
Supplier: Thermo Scientific™ K0512
Description
Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
- Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
- Fast—procedure takes approximately 20 minutes
- Universal—purifies genomic DNA from various sources
- Flexible—easy to scale up and down
- Safe—does not involve phenol extraction
Specifications
| • 40 mL Lysis Solution; room temperature • 8 mL Precipitation Solution (concentrated); room temperature • 10 mL NaCl Solution; room temperature |
|
| Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood | |
| gDNA | |
| Not High-throughput Compatible (Manual) | |
| Room Temperature | |
| Bacteria: ≤20 mg Cells: ≤106 Plant: ≤100 mg Tissue: ≤30 mg Serum: ≤200 μL Whole Blood: ≤200 μL |
| Organic Extraction | |
| 20 to 100 μL | |
| PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization | |
| 100 Preps | |
| 25 min. |
For Research Use Only. Not for use in diagnostic procedures.