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Thermo Scientific™ KpnI, HC (50 U/μL)
Thermo Scientific™ Fermentas KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion.
Supplier: Thermo Scientific™ ER0523
Description
Thermo Scientific™ KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion. More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.02μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with KpnI.
5'..G G T A CâµC...3'
3'..CâµC A T G G...5'
Conditions for 100% Activity:
- 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 0.02% Triton X-100 and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Methylation Effects:
- Dam: never overlaps —no effect
- Dcm: may overlap —no effect
- CpG: may overlap —no effect
- EcoKI: never overlaps —no effect
- EcoBI: never overlaps —no effect
Digestion of Agarose-embedded DNA:
- Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
Note:
High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
Specifications
| 50 U/μL | |
| Kpn I | |
| Yes | |
| No | |
| Restriction Enzyme |
| Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
| Unique Buffer (10X Buffer KpnI) | |
| 37°C | |
| HC; 20,000 U | |
| Traditional Cloning |
For Research Use Only. Not for use in diagnostic procedures.