Thermo Scientific™ Silica Bead DNA Gel Extraction Kit

Description

Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie , employing solubilization of the agarose gel and selective adsorption of nucleic acids on specially prepared silica particles at high salt concentration. Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water.

This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. Compared to column based gel-extraction kits, the DNA Gel Extraction Kit offers flexibility for scaling reaction and elution volumes up or down. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.

Highlights

• Flexible—easy to scale up or down both reaction and elution volumes
• Efficient—greater than 80% DNA recovery
• Fast—purification takes only 15 to 20 minutes
• Effective—suitable for DNA fragments as short as 100 bp
• Safe—does not involve phenol extraction