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Gibco™ Puromycin Dihydrochloride
Aminonucleoside antibiotic produced by Streptomyces alboniger
Supplier: Gibco™ A1113802
Description
Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.
- Used in cell biology as selection antibiotic in mammalian cell culture systems
- Recommended working concentration ranges from 0.2–5.0μg/mL
- Can be toxic to eukaryotic cells at concentrations as low as 1μg/mL
- Supplied at 10mg/mL in 20 mM HEPES buffer (pH 7.2–7.5)
Cell Culture, Cloning, Drosophila S2 Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation
Specifications
Storage conditions: -5 to -20°C Shipping conditions: Frozen Shelf life: 12 months from date of manufacture |
|
10 mg/mL | |
Liquid | |
Sterile-filtered | |
Eukaryotic Selection⁄Stable Cell Line Generation | |
1 bottLe |
Eukaryotic Cells, Prokaryotic Cells | |
Mammalian Cell Culture, Insect Cell Culture | |
Antibiotic | |
HEPES | |
Gibco™ | |
Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.