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Gibco™ Puromycin Dihydrochloride DFS Item

Aminonucleoside antibiotic produced by Streptomyces alboniger

Supplier:  Gibco™ A1113802

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Catalog No. A1113802


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Description

Description

Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

  • Used in cell biology as selection antibiotic in mammalian cell culture systems
  • Recommended working concentration ranges from 0.2–5.0μg/mL
  • Can be toxic to eukaryotic cells at concentrations as low as 1μg/mL
  • Supplied at 10mg/mL in 20 mM HEPES buffer (pH 7.2–7.5)

Cell Culture, Cloning, Drosophila S2 Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation

Specifications

Specifications

Storage conditions: -5 to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture
10 mg/mL
Liquid
Sterile-filtered
Eukaryotic Selection⁄Stable Cell Line Generation
20 mL
Eukaryotic Cells, Prokaryotic Cells
Mammalian Cell Culture, Insect Cell Culture
Antibiotic
HEPES
Gibco
Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.