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Gibco™ Ham's F-12 Nutrient Mix 
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.
Supplier: Gibco™ 11765054
Requires : Supplementation
Description
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red
The complete formulation is available.
Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Rat Astrocytes | |
| 1 X | |
| Ham's F-12 Nutrient Mixture | |
| Sterile-filtered | |
| No HEPES | |
| Gibco™ | |
| Room Temperature |
| CHO, COS-7, and rat prostate epithelial cells | |
| Animal Origin-free | |
| Liquid | |
| Sterile-filtered | |
| Glutamine, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |