Gibco™ HAT Supplement (50X)

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Supplier: Gibco™ 21060017

Catalog No. 21060017

Quantity

Description

Liquid mixture of sodium hypoxanthine (5mM), aminopterin (20μM) and thymidine (0.8mM)
Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT contributed by the fused spleen cell
Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway

Cell Culture, Mammalian Cell Culture

Shipping Condition: Dry Ice

Specifications

Content And Storage	Storage conditions: -5 to -20°C Shipping conditions: Dry ice Shelf life: 14 months from date of manufacture
Concentration	50 X
Product Type	HT Supplement
pH	6 to 8
Shipping Condition	Dry Ice

For Research Use Only. Not for use in diagnostic procedures.

Description