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Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
Supplier: Cytiva 28401353
Description
- Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
- One run in standard microcentrifuge takes approximatedly 10 min.
- Yields up to 750μg purified protein per column
- Allows direct purification of both clarified and unclarifed cell lysates
- Also useful for screening large numbers of small-scale bacterial lysates
- Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
Medium Characteristics:
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Stable (long-term) in the pH range 3-12
- Average particle size: 34μm
Specifications
| 100 μL | |
| 3 to 12 | |
| 4°C to 30°C | |
| Spin Column Kit |
| His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) | |
| 6% Highly Cross-linked Agarose | |
| 1 Pack His SpinTrap Spin Columns (50 x 100 μL) | |
| 600 μl |