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Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
Supplier: Cytiva 28932171
Description
- Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
- One run in standard microcentrifuge takes approximatedly 10 min.
- Yields up to 750μg purified protein per column
- Allows direct purification of both clarified and unclarifed cell lysates
- Also useful for screening large numbers of small-scale bacterial lysates
- Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
- Kit components (spin columns and buffer) can also be purchased separately
Medium Characteristics:
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Stable (long-term) in the pH range 3-12
- Average particle size: 34μm
Specifications
| His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) | |
| 2 to 14 | |
| 4°C to 30°C | |
| Spin Column Kit |
| 1 Pk. His SpinTrap (28-4013-53) and 1 Pk. His Buffer Kit (11-0034-00) | |
| 6% Highly Cross-linked Agarose | |
| 1 Piece |