Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Used for method optimization and parameter screening

Supplier: Cytiva 28978243

Catalog No. 45-002-546

Quantity

Description

Based on 6% highly cross-linked agarose with an average bead size of 90μm
Processed flow properties and high loading capacities
Bed height of 10cm is designed to allow method optimization and parameter screening
Easily connected in series to achieve 20cm bed height
Small bed volume gives fast results and minimal sample/buffer consumption
Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

Blue Sepharose

Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins

Ni Sepharose

Prepacked with Nickel Sepharose 6 Fast Flow
Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)

DEAE Sepharose

Specifications

Buffer	70% Ethanol, 6M Guanidine Hydrochloric, 8M Urea
Bed Volume	4.7mL
Diameter (Metric) Inner	7.7mm
Height (English)	3.93 in.
Flow Rate	<600cm/hr.
Ligand Type	Cibacron Blue (7μM/mL medium)
pH Range	4 to 12
Support Type	6% Cross-linked Agarose
Column Type	Prepacked Fast Flow Column

Product Type	HiScreen Blue Sepharose FF column
Diameter (English) Inner	0.3 in.
Quantity	1 x 4.7mL
Height (Metric)	100mm
Material	Polypropylene
Max. Pressure	1.5 bar
Particle Size	45 to 165μm
Content And Storage	4° to 8°C
For Use With (Application)	Purification of Albumin, Enzymes (including NAD+ and NADP+), coagulation facto rs, interferons and related Proteins

Description