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Cytiva HiScreen™ IMAC Sepharose™ 6 Prepacked Fast Flow Columns
Designed for method optimization and parameter screening
Supplier: Cytiva 28950517
Description
- Based on 6% highly cross-linked agarose with an average bead size of 90μm
- Processed flow properties with high binding and loading capacities
- Bed height of 10cm is designed to allow method optimization and parameter screening
- Optimal bead size for scale up
- Charge with the metal of choice for purification of histidine-tagged proteins
- Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity
- Bed volume: 4.7mL
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 90μm
- Max. flow velocity: <600cm/hr
- Material (Column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
Specifications
| 0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol | |
| 4.7mL | |
| 7.7mm | |
| 3.93 in. | |
| <600cm/hr. | |
| 1.5 bar | |
| 45 to 165μm | |
| 4° to 30°C | |
| Optimization and parameter screening |
| HiScreen IMAC IMAC Sepharose 6 FF column | |
| 0.3 in. | |
| 1 x 4.7mL | |
| 100mm | |
| Polypropylene | |
| 3 to 12 | |
| 6% Cross-linked Agarose | |
| Ion Exchange Column |