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Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns
Designed for method optimization and parameter screening
Supplier: Cytiva 28950515
Description
- Ready-to-use columns for convenient process development optimization and parameter screening
- Highly cross-linked agarose matrix with an average bead size of 34μm
- Bed height of 10cm designed to allow method optimization and parameter screening
- Easily connected in series to achieve 20cm bed height
- Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 34μm
- Max. flow velocity: <150cm/hr.
- Material (column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
- Flow velocity: <150cm/hr.
HiScreen Q HP
- Ionic capacity: 0.14 to 0.20mmol Cl-/mL
- Ligand: quaternary amine
- Ion exchanger type: strong anion exchanger
- Ionic capacity: 0.15 to 0.20mmol Cl-/mL
- Ligand: sulphopropyl
- Ion exchanger type: strong cation exchanger
Specifications
| 8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS | |
| 4.7mL | |
| 7.7mm | |
| 3.93 in. | |
| <150cm/hr. | |
| Sulphopropyl | |
| 4 to 13 | |
| 6% Cross-linked Agarose | |
| Ion Exchange Column |
| HiScreen SP Sepharose HP column | |
| 0.3 in. | |
| 1 x 4.7mL | |
| 100mm | |
| Polypropylene | |
| 3 bar | |
| 45 to 165μm | |
| 4° to 30°C | |
| Optimization and parameter screening, ion exchange chromato graphy |