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Cytiva HisPrep Column
Optimize purification of histidine-tagged recombinant proteins. Cytiva Life Sciences™ HisPrep Column is a ready to use column offering convenient scale-up purification of histidine-tagged proteins.
Supplier: Cytiva 28936551
Description
- Optimized for convenient scale-up purification of histidine-tagged proteins
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
- Negligible leakage of Ni2+
- Bed Volume 20ml
- High binding capacity
- Convenient and time-saving prepacked HiPrep format with precharged Ni Sepharose 6 Fast Flow medium
- Media:
- BioProcess media
- 90μm average particle size
- Metrix 6% cross-linked agarose
- pH stability working range 3-121
- Metal ion capacity ∽15μmol Ni2+™/ml medium
- Three years shelf life
- Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3
- Column:
- Inside Diameter is 16mm
- Material of Column hardware and tube is Polypropylene and bed support is Polymethyl-Methacrylate
- Bed support Filter
- Bed Dimensions 16 x 100mm
- Flow rate <10ml/min1
- Flow Velocity <300cm/h
- Pressure Max. 1.5 bar
Specifications
| 0.6 in. | |
| 4°C to 30°C | |
| 20 mL |
| <10 mL/min. | |
| 1.6 cm | |
| Each |