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Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
$97.75 - $1135.47
Specifications
| Material | Polypropylene |
|---|---|
| pH Range | 3 to 12 |
| Support Type | 6% Cross-linked Agarose |
| Content And Storage | 4°C to 30°C |
| Particle Size | 45 to 165 μm |
| Catalog Number | Mfr. No. | Quantity | Flow Rate | Price | Quantity | ||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Flow Rate | Price | Quantity | ||||
|
45-000-335
|
Cytiva
17528601 |
5 x 5 mL | <20 mL/min. |
Pack of 5 Each for $1,135.47
|
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|
45-000-000
|
Cytiva
11000458 |
60 mL | <4 mL/min. |
Pack of 5 Each for $314.36
|
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|
45-000-507
|
Cytiva
29048631 |
1 mL | <4 mL/min. |
Each for $97.75
|
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Description
- Prepacked with Ni Sepharose™ 6 Fast Flow media
- Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
- 1 and 5mL columns
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 90μm
- pH stability working range: 3 to 12
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
5mL columns
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifications
| Polypropylene | |
| 6% Cross-linked Agarose | |
| 45 to 165 μm |
| 3 to 12 | |
| 4°C to 30°C | |
| Pre-packed Column |