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Cytiva HiTrap™ AVB Sepharose™ Columns
Enables purification of adeno associated viruses (AAV) in one step, with high purity and excellent yields. Cytiva HiTrap™ AVB Sepharose™ Columns are pre-packed with affinity chromatography medium (resin) for simple operation with a syringe, pump or chromatographic system.
Supplier: Cytiva 28411212
Description
AVB Sepharose High Performance is an affinity resin designed for the purification of adeno associated virus (AAV). It enables purification of adeno associated viruses in one step with high purity and excellent yields.- One step purification of adeno associated viruses
- Affinity resin with high selectivity and low background binding
- Large surface area to give enhanced capacity
- Prepacked HiTrap columns for simple operation with a syringe, pump, or chromatographic system
- It is expected to have affinity for most of the other adeno associated virus subclasses
- The ligand is a 14 kD fragment from a single chain antibody, expressed in yeast
Specifications
1 x 5 mL | |
Stable to all commonly used aqueous buffers | |
>10â¿12 genecopies/mL Chromatography Mediμm | |
16 mm | |
24 to 44 μm | |
Polypropylene | |
Cross-linked agarose | |
5 mL |
One Step Purification of Adeno Associated Viruses | |
Store prepacked column at 4°C to 30°C in 20% Ethanol and Media at 4°C to 8°C in 20% Ethanol. | |
HiTrap AVB Sepharose HP Column | |
<150 cm/hr | |
25 mm | |
70 psi (5 bar) | |
3 to 10 | |
14 kD recombinant protein |