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Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Supplier: Cytiva 17040903
Description
- Uses different kinds of metal ions
- Binding capacity ∽12mg histidine-tagged protein/mL medium
- Metal ion capacity ∽23μmol Cu2+/mL medium
- Prepacked with Chelating Sepharose High Performance media
- Iminoacetic acid coupled to a 6% agarose matrix by ether linkage
Specifications
| 6M Guanidine Hydrochloride, 8M Urea | |
| 5mL | |
| 16mm | |
| 0.98 in. | |
| <20 mL/min. | |
| Iminodiacetic Acid | |
| 3 to 13 | |
| 6% Highly Cross-linked Agarose | |
| Affinity Column |
| HiTrap chelating HP column | |
| 0.62 in. | |
| 5 x 5mL | |
| 25mm | |
| Polypropylene | |
| 3 bar | |
| 34μm | |
| 4° to 30°C | |
| IMAC and subsequent purification of Polyhistidine tagged Proteins |