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Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Supplier: Invitrogen™ IOCLB1
Description
- The 5x Cell Lysis Mix is used to lyse cells in the presence of culture medium and is typically used to lyse non-adherent cells
- Dilute 5x Cell Lysis Mix 5-fold with water to make a 1x Cell Lysis Mix
- 1x Mix is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation
- Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required
- Supplementing Cell Lysis Mix with extra components (e.g. protease inhibitors, chelating agents, detergents) should be tested on a case-by-case basis for compatibility with InstantOne ELISA assays
- Included in the InstantOne ELISA kits
Specifications
| ELISA Assay/Lysis Buffer | |
| 100 mL | |
| Liquid | |
| ELISA |
For Research Use Only. Not for use in diagnostic procedures.