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Invitrogen™ Calcium Phosphate Transfection Kit 
Provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation
Supplier: Invitrogen™ K278001
Includes: 2 x 12mL Tissue Culture Sterile Water, 2 x 12mL 2X HEPES Buffered Saline (HBS), 3 x 1mL 2M CaCl2, 20μg pcDNA™3.1/His/lacZ
Description
- Based on forming a calcium phosphate-DNA precipitate
- Facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis
- First developed by Graham and van der Ebb and was later modified by Wigle
- Used to transfect a wide variety of cell types for transient expression or for producing stable transformants
- DNA is mixed directly with a concentrated solution of CaCl2, which is then added dropwise to a phosphate buffer to form a fine precipitate
- Aeration of the phosphate buffer while adding the DNA-CaCl2 solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently
- Product size: 75 reactions
Cell Culture, Plasmid Transfection, Transfection
Order Info
Shipping Condition: Room Temperature
Specifications
| 2 × 12 ml Tissue Culture Sterile Water 2 × 12 ml 2X HEPES Buffered Saline (HBS) 3 × 1 ml 2 M CaCl2 20 μg pcDNA™3.1/His/lacZ Store all components at -5 to -30°C. |
|
| 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks | |
| Chemical Transfection | |
| Not High-throughput Compatible (Manual) | |
| 75 Reactions |
| Established Cell Lines | |
| Plasmid DNA | |
| Transfection | |
| Transfection Kit | |
| No |
For Research Use Only. Not for use in diagnostic procedures.