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Invitrogen™ DNA Polymerase I 
DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities
Supplier: Invitrogen™ 18010017
Description
Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definition
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Unit reaction conditions
50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.
- Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
- Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid
Cloning, Restriction Enzyme Cloning
Specifications
| Store in freezer (-5 to -30°C). | |
| Liquid | |
| 3' - 5', 5' - 3' | |
| Wet Ice |
| DNA Polymerase | |
| DNA Polymerase I | |
| 250 U | |
| No |
For Research Use Only. Not for use in diagnostic procedures.