Invitrogen™ DNA Polymerase I/DNase I

An optimized mixture of both enzymes for efficient nick translation of DNA

Supplier: Invitrogen™ 18162016

Catalog No. 18162016

Quantity

Includes: DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.

Description

Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
Performance and Quality Testing: Performance tested in nick translation reaction
Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl₂ , 1mM 2-mercaptoethanol, 80μg/mL template•primer, 32μM dTTP, 69nM [^{3H]dTTP, and enzyme in 100μL for 30 min. at 37°C}

Cloning, Restriction Enzyme Cloning

Shipping Condition: Wet Ice

Specifications

Content And Storage	DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.
Polymerase	DNA Polymerase I
Quantity	250 U
Hot Start	No

For Research Use Only. Not for use in diagnostic procedures.

Description