Invitrogen™ Dynabeads™ kilobaseBINDER™ Kit 

Description

The Dynabeads kilobaseBINDER Kit is designed for immobilizing linear, biotinylated, long double-stranded (ds) DNA molecules (>2 kb). It combines streptavidin-coupled Dynabeads and a unique binding solution containing a patented immobilization activator that significantly increases the binding capacity for linear, large dsDNA fragments. This product provides an advantage whenever immobilization of a large quantity of long, biotinylated dsDNA is required.

Benefits of Dynabeads kilobaseBINDER Kit include:
• Performance—high binding capacity for >2 kb dsDNA molecules
• Efficiency—fast kinetics allows the isolation of chromatin within minutes
• Consistency—high batch-to-batch reproducibility secures consistent results
• Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System

Consistency and reproducibility  
The monolayer of streptavidin leaves most of the biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.

Binding capacity
The size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. Due to steric hindrance, the binding efficiency is significantly reduced when the fragment size of nucleic acid exceeds 2 kb. The Dynabeads kilobaseBINDER Kit activates long, linear dsDNA, neutralizing steric hindrance and facilitating binding to the bead surface. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads kilobaseBINDER beads typically binds:
• ∼70 pmoles of a 4 kb biotinylated DNA fragment
• ∼80 pmoles of a 10 kb fragment per mg beads
• >195 μg (6 pmol) biotinylated 50 kb DNA fragment per mg beads

The binding solution increases the binding capacity of biotinylated 4 kb DNA fragments >10 times compared to not using the binding solution.

Applications
This product is useful for applications in cell biology (e.g. building chromatin beads that mimic chromosomes for the study of self-organization of microtubules into bipolar spindles, building synthetic nuclei for studying RNA export from the nucleus in vitro, reconstituting nuclear movement along microtubules and examination of the molecular basis of this movement in vitro).

The kit is also suitable for efficient target enrichment for long-read sequencing. We recommend extending the biotinylated probe by PCR after target hybridization to help ensure the resulting long DNA fragment is double-stranded.

Automation ready with the KingFisher Purification System 
The 2.8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.

Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads kilobaseBINDER Kit in a commercial product or service or need a larger bulk volume of this product, please contact us atoemdynal@lifetech.com or see our Dynabeads OEM page.

Order Info

Shipping Condition: Room Temperature