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Invitrogen™ MAX Efficiency™ DH5α Competent Cells 
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
Supplier: Invitrogen™ 18258012
Includes: Max Efficiency DH5α Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, S.O.C. medium: 2 bottles, 6mL each
Description
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
Benefits of MAX Efficiency DH5α Competent Cells:
• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid libraries
DH5α competent cells for high transformation efficiency
The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (Cat. No. 18288019) (or similar) is provided to allow plaque formation.
Features of MAX Efficiency DH5α Competent Cells include:
• Transformation efficiency of >1 x 109 transformants/μg plasmid DNA
• High plasmid yield from the DH5α (endA1) E. coli strain
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)
Genotype:
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1
Find the strain and format that fits you needs
• DH5α strain and its derivatives available in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats.
• ElectroMAX™ DH5α-E Competent Cells (Cat. No. 11319019) are engineered for electroporation to achieve high transformation efficiency of >1 x 1010.
• DH5α T1R competent cells are available in MultiShot format for high throughput applications.
Order Info
Shipping Condition: Dry Ice
Specifications
| Chemically Competent Cells | |
| Yes (endA1) | |
| High Efficiency (>1 x 109 cfu/μg) | |
| No | |
| Yes (lacZΔM15) | |
| No | |
| Dry Ice | |
| E. coli (K12) | |
| MAX Efficiency™ |
| No | |
| No | |
| • Max Efficiency DH5α Competent Cells (5 x 200 μL) Store Competent Cells at –80°C. • pUC19 DNA (100 μL at 0.01 μg/mL) Store pUC19 DNA at –20°C. • S.O.C. Medium (2 x 6 mL) Store S.O.C. Medium at 4°C or room temperature. |
|
| Not suitable for cloning unstable DNA | |
| Low | |
| Yes (recA1) | |
| No | |
| Tube | |
| 5 x 200 μL |
For Research Use Only. Not for use in diagnostic procedures.