Invitrogen™ Platinum™ Taq DNA Polymerase 

Description

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

• Convenient reaction assembly at room temperature
• 5' to 3' exonuclease activity
• Lacks 3' to 5' exonuclease activity

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• Hot-start PCR
• RT-PCR

Notes

• For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
• Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.