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Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
Supplier: Invitrogen™ 10966083
Description
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The 'hot start' property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.
Using Platinum Taq DNA Polymerase
The hot-start property of Platinum Taq DNA Polymerase allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.
Note: For superior PCR performance, we recommend next-generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
Specifications
• Platinum Taq DNA Polymerase, 1000 μL • 10X PCR Buffer (no magnesium), 50 mL • 50 mM MgCl2, 10 mL • KB Extender, 10 mL Store at -10°C to -30°C in a non-frost-free freezer. |
|
Low | |
Standard | |
DNA Polymerase | |
Hot-start PCR | |
Built-In Hot Start | |
3'-A | |
5 kb or less |
Tube | |
Platinum Taq DNA Polymerase | |
5' - 3' | |
Dry Ice | |
1X | |
5000 Reactions | |
Separate Components | |
DNA |
For Research Use Only. Not for use in diagnostic procedures.