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Invitrogen™ Platinum™ PCR SuperMix High Fidelity 
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Supplier: Invitrogen™ 12532016
Description
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.
High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.
Benefits of Platinum PCR SuperMix, High Fidelity
- Six-times higher fidelity than Taq DNA polymerase
- Amplifies fragments up to 15 kb
- Minimizes PCR optimization
- Room-temperature reaction assembly
Applications
- High-fidelity PCR
- Cloning
- Mutagenesis
Notes
- Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
- Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
Specifications
| Platinum PCR SuperMix High Fidelity (4 x 1.125 mL) Store at -20°C in a non-frost-free freezer. |
|
| Platinum Taq DNA Polymerase High Fidelity | |
| 100 rxns | |
| Hot-start PCR, High-fidelity PCR | |
| 6X | |
| 100 Reactions | |
| SuperMix or Master Mix |
| Low | |
| Standard | |
| Wet or Dry Ice | |
| 1.1X | |
| Built-In Hot Start | |
| Mixed | |
| 15 kb or less |
For Research Use Only. Not for use in diagnostic procedures.