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Invitrogen™ PureLink™ Genomic DNA Mini Kit 
The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.
Supplier: Invitrogen™ K182002
Description
The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:
• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity
Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.
Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.
Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine.
Specifications
| • 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature • 45 mL PureLink Genomic Digestion Buffer; room temperature • 50 mL PureLinkGenomic Wash Buffer 1; room temperature • 37.5 mL PureLink Genomic Wash Buffer 2; room temperature • 50 mL PureLink Genomic Elution Buffer; room temperature • 5 mL RNase A (20 mg/mL); room temperature • 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage • 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature • 5 x 100 PureLink Collection Tubes; room temperature |
|
| Mini | |
| 25 to 200 μL | |
| PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization | |
| 250 Preps | |
| Up to 15 min. after cell lysis | |
| Bacteria: ≤109 cells Cells: ≤106 Tissue: ≤25 mg Mouse Tail: 1 cm Rat Tail: 0.5 cm Whole Blood: ≤1 mL Yeast: ≤107 cells FFPE: ≤8 sections of 5-15 μM thickness Oragene™ Preserved Saliva: ≤4 mL Blood Spots: ≤5 punches |
| Silica Spin Column | |
| Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast | |
| gDNA | |
| Not High-throughput Compatible (Manual) | |
| Room Temperature | |
| ≤40 μg |
For Research Use Only. Not for use in diagnostic procedures.