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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit DFS Item

The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.

Supplier:  Invitrogen™ K210011

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Catalog No. K210011


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Description

Description

The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns. Features of the PureLink™ Quick Plasmid Miniprep Kit:

  • Fast—ready-to-use plasmid DNA typically in about 30 min
  • Flexible—formats work with vacuum and centrifugation platforms

Reliable and cost-effective isolation of plasmid DNA
The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats
The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications
Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.

Specifications

Specifications

20 mg/mL
• Resuspension buffer
• RNase A (20 mg/mL)
• Lysis buffer
• Precipitation buffer
• Wash buffers
• TE buffer
• Spin columns in wash tubes
• Elution tubes

Silica Spin Column
Bacterial Culture
Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
250 Preps
PureLink™
250 Preps
Plasmid DNA
Spin Column
Spin Column
Mini
Plasmid DNA
Not High-throughput Compatible (Manual)
< 100 μg (Small-Scale) Plasmid DNA
Quick Plasmid MiniPrep Kit
Room Temperature
30 to 45 min.
SDS
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For Research Use Only. Not for use in diagnostic procedures.