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Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit 
The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants.
Supplier: Invitrogen™ 12280050
Includes:
- 32mL Viral Lysis buffer (L22)
- 15mL 5X Wash Buffer (W5)
- 2 x 1.6mL Proteinase K (20mg/mL in storage buffer)
- 310μg lyophilized Carrier RNA
- 15.5mL Sterile, RNAase-free water
- 50 Viral Spin Columns with Collection Tubes
- 2 x 50 Wash Tubes (2.0mL)
- 50 Recovery Tubes (1.5mL)
Description
The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.
The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.
The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis
Order Info
Shipping Condition: Room Temperature
Specifications
| Spin Column | |
| Silica Spin Column | |
| Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM) | |
| Viral RNA and DNA | |
| Not High-throughput Compatible (Manual) | |
| Room Temperature | |
| 5 μg (Binding capacity) |
| • 310 μg Carrier RNA; –20°C • 32 mL Viral Lysis Buffer L22; room temperature • 15 mL Wash Buffer WII; room temperature • 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage • 15.5 mL RNase-free Water E3; room temperature • 50 Viral Spin Columns with Collection Tubes; room temperature • 2 x 50 Wash Tubes; room temperature • 50 Recovery Tubes; room temperature |
|
| Mini | |
| 10 to 50 μL | |
| RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization | |
| 50 Preps | |
| 45 min. | |
| ≤500 μL |
For Research Use Only. Not for use in diagnostic procedures.