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Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor 
Protects mRNA and improves total cDNA yields
Supplier: Invitrogen™ 10777019
Description
RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of approx. 52 kDa. RNaseOUT inhibits RNase A, RNase B, and RNase C.
Applications cDNA synthesis, RT-PCR, and in vitro transcription and translation
Source Purified by affinity chromatography from E. coli expressing a cloned porcine gene
Performance and quality testing SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR
Unit definition One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate
Unit reaction conditions 100mM Tris-acetate (pH 6.5), 1mM EDTA, 0.2mM cCMP, 2 μg RNase A in 1mL from 0 to 10 min at 25°C
PCR and Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Dry ice
Specifications
| 100 mM | |
| 10 min. | |
| 5000 units |
| Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity. The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation. |
|
| RNaseOUT™ | |
| Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.