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Invitrogen™ T4 DNA Ligase (5 U/μL) 
Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini
Supplier: Invitrogen™ 15224041
Includes: T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000].
Description
The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.
Unit Definition: One unit catalyzes the exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)
Unit Reaction Conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2 , 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C.
Order Info
Shipping Condition: Dry Ice
Specifications
| 5 U/μL | |
| Dry Ice | |
| 5X Reaction Buffer | |
| T4 DNA Ligase |
| T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C. | |
| Ligase | |
| 250 U |
For Research Use Only. Not for use in diagnostic procedures.