Thermo Scientific™ MagnaBind™ Protein A Beads

Description

Thermo Scientific™ MagnaBind Magnetic Beads are available with streptavidin, Protein A, Protein G, GAM IgG, GAR IgG and other ligands for use in small-scale benchtop magnetic affinity protocols.

MagnaBind Magnetic Beads enable small-scale affinity purification and cell-separation without columns or centrifugation. The beads are silanized iron oxide magnetic particles activated with various affinity ligands. After a binding step in an affinity purification procedure, the magnetic particles are easily and rapidly collected by placing the microcentrifuge tube or reaction vessel next to an appropriate rare-earth magnet. MagnaBind Beads respond rapidly to MagnaBind Magnets. The particles can be easily dispersed and regathered multiple times (i.e., they will not irreversibly aggregate) because they do not have any magnetic memory.

MagnaBind Beads are available pre-coated with Protein A, Protein G, streptavidin, anti-mouse or anti-rabbit antibodies. Also available are beads activated with either amine or carboxyl groups as targets for customized covalent immobilization other proteins or ligands.

Highlights:

• Available pre-coated with popular affinity ligands or derivatized for covalent attachment of proteins and other specific ligands
• Beads do not irreversibly aggregate because they have no magnetic memory; collect and disperse the beads multiple times if needed
• Most separations require a short five- to 10-minute benchtop procedure

Properties of underivatized MagnaBind Beads:

• Composition: silanized iron oxide
• Magnetization: 25 to 35 EMU/g
• Type of Magnetization: superparamagnetic (no magnetic memory)
• Surface Area: greater than 100m²/g
• Bead Size: 1 to 4μm diameter
• Settling Rate: 4% in 30 minutes
• Effective Density: 2.5g/mL
• Number of Beads: 1 x 10⁸ beads per milliliter
• pH Stability: aqueous solution, above pH 4.0
• Concentration: 5mg/mL

Requires:

To remove MagnaBind Beads from suspension, an external magnetic field is used; see the ordering table for a selection of magnets.

Recommended for:

Cell sorting using either positive or negative selection; Protein purification or immunoassays using either direct or indirect methods; Immunoprecipiation