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  8. 11090099

Gibco™ MEM, no glutamine DFS Item

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells.

Supplier:  Gibco™ 11090099

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Catalog No. 11090099


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Description

Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Specifications

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
MEM (Minimum Essential Medium)
Sterile-filtered
No Glutamine, No HEPES, No Sodium Pyruvate
Gibco™
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Animal Origin-free
Liquid
Standard Serum Supplementation
Low Glucose, Phenol Red
cGMP-compliant under the ISO 13485 standard
10 x 500 mL
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.