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Invitrogen™ Rhodamine Phalloidin DFS Item

High-affinity F-actin probe conjugated to red-orange fluorescent dye, tetramethylrhodamine (TRITC)

Supplier:  Invitrogen™ R415

Catalog No. R415


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Description

Description

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies.

  • Selectively stains F-actin
  • Excitiation/Emission: 540/565nm
  • Superior to antibody staining
  • Optimal for fixed and permeabilized samples
  • Very widely cited fluorescent phalloidin conjugate

Cell Analysis, Cell Structure, Cytoskeleton

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Store in freezer -5°C to -30°C and protect from light.
TRITC (Tetramethylrhodamine Isothiocyanate)
Room Temperature
Phalloidin
540⁄565
300 units
Classic Dyes
Actin, Cytoskeleton
SDS
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For Research Use Only. Not for use in diagnostic procedures.