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Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
$131.04 - $7909.97
Specifications
| Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
|---|---|
| Product Type | Ni Sepharose 6 FF chromatography media |
| Height (English) | 9.84 in. |
| Height (Metric) | 250mm |
| Flow Rate | 600cm/hr. |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
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45-002-987
|
Cytiva
17531806 |
5mL |
Each for $131.04
|
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45-002-984
|
Cytiva
17531801 |
25mL |
Each for $539.23
|
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|
45-002-985
|
Cytiva
17531802 |
100mL |
Each for $1,839.54
|
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|
45-002-986
|
Cytiva
17531803 |
500mL |
Each for $7,909.97
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Description
Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
- Designed for scaling up purification of histidine-tagged proteins.
- High protein binding capacity and minimal leakage of Ni2+ ions.
- As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep and HisTrap column formats.
- Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
- The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
- Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
- As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Specifications
| 0.1M HCl, 0.1M NaOH, 8M Urea | |
| 9.84 in. | |
| 600cm/hr. | |
| 3 to 12 | |
| 6% Cross-linked Agarose | |
| Ni-Sepharose |
| Ni Sepharose 6 FF chromatography media | |
| 250mm | |
| 1 bar | |
| 90μm | |
| 4° to 30°C |