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Gibco™ Opti-MEM™ I Reduced Serum Medium 
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Supplier: Gibco™ 31985070
Description
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.
This Opti-MEM™ I is modified as follows:
With: L-glutamine, Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
| Store at 2–8°C and protect from light. | |
| Primary Fibroblasts | |
| 1 X | |
| Opti-MEM I | |
| Sterile-filtered | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
| Sp2, AE-1, CHO, BHK-21, and HEK | |
| Human Origin | |
| Liquid | |
| Sterile-filtered | |
| Glutamine, Phenol Red | |
| Gibco™, Opti-MEM™ | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.